Strong Cation Exchanger: SP 1500 and SP 3000
SP 1500 is prepared from 10 µm particles of highly cross-linked PS-DVB beads with pore size of 500 Å. Similarly, SP 3000 is produced from 10 µm particles of highly cross-linked PS-DVB beads with pore size of 1500 Å. After being coated with neutral, hydrophilic layer, the polymer is chemically bonded with the polymer chains of sulfopropyl (SP) groups to provide the optimum density of strong cation exchange functionalities for macromolecule separations.
The media are compatible with aqueous solvents in the pH range of 1 to 14, as well as with most organic solvents. Elution of proteins can be achieved at relatively low ionic strength (< 0.5 M NaCl) in 15 minutes at a flow rate of 1.0 mL/min. Because of the hydrophilic nature of the adsorbents, proteins with pI value close to or below the mobile phase pH will not be retained on Hydrocell cation exchange columns.