Hydrocell Ion Exchange Columns
Ion Exchange Columns
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HYDROCELL ion exchange adsorbents were developed by BioChrom Labs, Inc. for biomolecule separations, including proteins, peptides, oligonucleotides, and DNA fragments. They are based on the coatings of the neutral hydrophilic layer on the rigid macroporous polystyrene-divinylbenzene (PS-DVB) matrix, followed by chemical immobolization of the polymer chains of bonded phase on the hydrophilic surface.
Unlike some columns where hydrophobic retention can complicate ion exchange chromatography by producing irreversible adsorption, HYDROCELL ion exchangers are very hydrophilic. Elution of proteins can be achieved with buffers of moderate ionic strength, typically less than 0.5M NaCl, in 15 minutes at a flow rate of 1.0 mL per minute.
The packings do not have any weak ester or amide linkages as do other polymeric gels. Their chemical and physical stabilities allow rapid equilibration and fast clean up using high salt, HCl, NaOH and organic solvents.
With a pH range of 1 to 14 and pressure limit of 4000 psi, HYDROCELL columns are ideal for the separation and analysis of a wide range of biological materials.