Hydrophobic Interaction Columns
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Hydrophobic Interaction Chromatography (HIC) separates proteins by mechanisms similar to Reversed-Phase Chromatography but under gentle reverse salt gradient elution conditions in aqueous buffers. Since no organic solvent is used, biological activity has a much higher probability of being retained.
The Major Advantages of HYDROCELL Hydorphobic Interaction Columns are:
- Retain Biological Activity
- High Resolution & Efficiency
- Excellent Protein Recoveries
HYDROCELL C3 1500, C4 1500 and Phenyl 1500 are prepared from 10 µm particles of hydrophilic PS-DVB beads with pore diameter of 500 Å. Similarly, C3 3000, C4 3000 and Phenyl 3000 are produced from 10 µm particles of hydrophilic, macroporous PS-DVB beads with average pore diameter of 1500Å.
The packing materials have been chemically bonded with allyl (C3), butyl (C4) or phenyl as a hydrophobic group. These packings are the same as HYDROCELL ion exchange packings, which do not have any weak ester linkages as do the other commercial products.
They are stable in a pH range of 1 to 14 and are compatible with most organic solvents. The columns can be operated at high flow rates for rapid equilibration and fast clean up.